Giemsa Staining Solution Instruction Manual
Supplier: Shanghai Yuanmu Biotechnology Co., Ltd.
Product Name: Giemsa Working Solution
English Name: Giemsastain
Specification: 100ml / 500ml
Storage: The Giemsa dye solution has a shelf life of at least three years when stored at room temperature. The working solution is ready to use and should be stored at 2–8°C for up to one month.
Introduction to Giemsa Working Solution:
Giemsa staining solution contains a combination of azure and eosin. Different cellular components have distinct chemical properties and varying affinities for dyes. For instance, eosinophilic granules, which are basic proteins, bind with the acidic dye eosin and appear pink. On the other hand, basophilic components such as nucleoproteins or lymphocyte cytoplasm, being acidic, combine with basic dyes like methylene blue, resulting in a purple-blue color. Neutral components, which are in an isoelectric state, can bind with both eosin and methylene blue, appearing lavender.
The ability of Giemsa dye to differentiate various cell structures depends on their interactions with different dyes. The Giemsa staining solution produced by Yuanmu Bio is made using imported Giemsa dye, ensuring that nuclei stain purple or blue-violet, while the cytoplasm appears pink. Under a light microscope, the stained cells show clear and distinct images. This solution is commonly used to visualize differences in shape and size among various blood cells in blood smears, offering excellent staining results, strong staining power, and vibrant coloration.
Giemsa Staining Procedure (for reference only):
To prepare the working solution, mix 1ml of Giemsa Concentrate with 9ml of Giemsa Diluent and use immediately.
1. Prepare a blood smear using standard methods. Allow the smear to air dry, then fix it in methanol for 2–3 minutes. Ensure the smear is evenly distributed and of appropriate thickness to avoid affecting the staining outcome.
2. Place the blood or bone marrow smear on a staining rack, add the diluted staining solution to cover the entire smear, and incubate at room temperature for 15–30 minutes.
3. Gently rinse the slide from one end with tap water, avoiding direct pouring onto the smear. After rinsing, allow the slide to air dry before examining under a microscope.
Important Notes:
1. For your safety, always wear a lab coat and disposable gloves when handling the solution.
2. If the staining is too dark or too light, adjust the staining time or the concentration of the working solution accordingly.
3. The stock solution should be prepared using conventional techniques and filtered through filter paper. Avoid contact with water during use, as this may reduce its effectiveness.
4. pH plays a critical role in cell staining. Ensure that all slides are clean and free from acid or alkali contamination to maintain consistent staining results.
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